As single-molecule localization microscopy (SMLM) achieves higher precision, linkage errors from antibody labeling increasingly hinder imaging accuracy. Here, the authors employ MINFLUX to analyze linkage error distributions across various antibody labeling strategies, revealing deviations from normality and suggesting statistical methods to enhance SMLM accuracy, with implications for super-resolution imaging advancements.
- Jing Wang
- Huihui Zou
- Min Gu
