Nature Search

Engineering eukaryotic transposon-encoded Fanzor2 system for genome editing in mammals

By engineering the eukaryotic-derived Fanzor2-ωRNA system, Wei, Gao, Pan, Li and Chen developed a compact genome editor enNlovFz2, which demonstrated efficient genome-editing activity in vitro and in vivo, with a broad target-adjacent motif scope.

Yinghui Wei
Pengfei Gao
Xiaolong Wang
Research20 May 2025
Nature Chemical Biology

P: 1-10
Author Correction: Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation

Guoling Li
Xue Dong
Yingsi Zhou
Amendments and CorrectionsOpen Access18 Oct 2024
Nature Communications

Volume: 15, P: 1
Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation

Engineered TadA variants in cytosine base editors (CBEs) offer advantages like smaller size and reduced off-target effects. Here, authors develop an advanced CBE (aTdCBE) through ortholog screening and protein engineering, successfully enabling exon skipping in Duchenne muscular dystrophy model.

Guoling Li
Xue Dong
Yingsi Zhou
ResearchOpen Access16 Sept 2024
Nature Communications

Volume: 15, P: 1-10
Engineered IscB–ωRNA system with expanded target range for base editing

Xiao et al. characterize 19 previously unidentified IscB proteins, 10 of which show activity in mammalian cells. They engineer one of the proteins into a robust in vivo genome-editing tool with a broad target-adjacent motif scope and further develop it into a base editor.

Qingquan Xiao
Guoling Li
Hainan Zhang
ResearchOpen Access15 Aug 2024
Nature Chemical Biology

Volume: 21, P: 100-108
Engineering a transposon-associated TnpB-ωRNA system for efficient gene editing and phenotypic correction of a tyrosinaemia mouse model

Miniature gene editing tools are highly desired for efficient in vivo delivery and disease treatment. Here, the authors reported engineering hypercompact TnpB-ωRNA for robust gene editing with minimal off-target effect in cultured cells and use it to rescue fatal genetic liver disease in a tyrosinaemia mouse model.

Zhifang Li
Ruochen Guo
Chunlong Xu
ResearchOpen Access27 Jan 2024
Nature Communications

Volume: 15, P: 1-11
An RNA editing strategy rescues gene duplication in a mouse model of MECP2 duplication syndrome and nonhuman primates

Duplication of the MECP2 gene (encoding methyl-CpG-binding protein 2) causes MECP2 duplication syndrome. Here the authors develop a Cas13Y system capable of targeting the MECP2 mRNA for degradation and reducing protein levels in the brain of humanized MECP2-transgenic mice and nonhuman primates.

Dong Yang
Xiaoqing Wu
Hui Yang
Research12 Dec 2024
Nature Neuroscience

Volume: 28, P: 72-83
Development of deaminase-free T-to-S base editor and C-to-G base editor by engineered human uracil DNA glycosylase

Efficient base editors for direct thymine (T) editing are highly desirable. Here, authors develop two deaminase-free glycosylase-based base editors for direct T editing (gTBE) and C editing (gCBE) by rounds of structure-informed mutagenesis on human DNA glycosylase UNG and further engineering.

Huawei Tong
Haoqiang Wang
Hui Yang
ResearchOpen Access08 Jun 2024
Nature Communications

Volume: 15, P: 1-12
Identification of a novel ANO5 missense mutation in a Chinese family with familial florid osseous dysplasia

Mingming Lv
Guoling You
Jian Sun
Research17 Apr 2019
Journal of Human Genetics

Volume: 64, P: 599-607
Engineered CRISPR-OsCas12f1 and RhCas12f1 with robust activities and expanded target range for genome editing

Cas12f proteins are small and sought after for therapeutic applications. Here the authors report six bacterial Cas12f1 proteins with nuclease activity in mammalian cells, perform sgRNA and protein engineering to generate variants with enhanced editing and broader PAMs, apply an inducible version in vivo.

Xiangfeng Kong
Hainan Zhang
Hui Yang
ResearchOpen Access11 Apr 2023
Nature Communications

Volume: 14, P: 1-13
Adenine base editing-mediated exon skipping restores dystrophin in humanized Duchenne mouse model

Duchenne muscular dystrophy (DMD) results from mutations in the DMD. Here the authors report using adenine base editing of exon splice sites to restore dystrophin expression and muscle function in a humanized mouse model of DMD with exon 50 and 51 of mouse DMD gene replaced with human exon 50.

Jiajia Lin
Ming Jin
Guoling Li
ResearchOpen Access15 Jul 2024
Nature Communications

Volume: 15, P: 1-12
C-JUN overexpressing CAR-T cells in acute myeloid leukemia: preclinical characterization and phase I trial

Chimeric Antigen Receptor (CAR)-T cells show suboptimal efficacy in Acute Myeloid Leukaemia (AML). Here, the authors show that overexpression of C-JUN improves CAR-T cells efficacy in preclinical models for AML and report preliminary results of a pilot phase I clinical trial.

Shiyu Zuo
Chuo Li
Xiaoming Feng
ResearchOpen Access22 Jul 2024
Nature Communications

Volume: 15, P: 1-20
High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects

Several engineered Cas13 variants achieve targeted RNA degradation with minimal collateral effects.

Huawei Tong
Jia Huang
Hui Yang
Research11 Aug 2022
Nature Biotechnology

Volume: 41, P: 108-119
Development of miniature base editors using engineered IscB nickase

The miniature RNA-guided endonuclease IscB and its ωRNA were engineered for efficient gene editing in mammalian cells. Fusions of ‘enIscB’ to T5 exonuclease and cytosine or adenosine deaminase yield versatile tools for genome engineering.

Dingyi Han
Qingquan Xiao
Yingsi Zhou
Research25 May 2023
Nature Methods

Volume: 20, P: 1029-1036
Enhanced pericyte-endothelial interactions through NO-boosted extracellular vesicles drive revascularization in a mouse model of ischemic injury

Treatment alternatives are not available for a significant portion of critical limb ischemia patients. Here, the authors show a strategy to engineer mesenchymal stem cells-derived extracellular vesicles to induce revascularization in mice.

Ling Guo
Qiang Yang
Min Feng
ResearchOpen Access13 Nov 2023
Nature Communications

Volume: 14, P: 1-18
Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells

Guoling Li
Xianwei Zhang
Zhenfang Wu
ResearchOpen Access21 Aug 2017
Scientific Reports

Volume: 7, P: 1-11
In vivo treatment of tyrosinaemia with hypercompact Cas12f1

Ruochen Guo
Zhifang Li
Chunlong Xu
CorrespondenceOpen Access18 Jul 2023
Cell Discovery

Volume: 9, P: 1-4
Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors

Fang Zeng
Zicong Li
Zhenfang Wu
ResearchOpen Access24 Jan 2017
Scientific Reports

Volume: 7, P: 1-13
IL-37bΔ1-45 suppresses the migration and invasion of endometrial cancer cells by targeting the Rac1/NF-κB/MMP2 signal pathway

IL-37 protein is downregulated in endometrial carcinoma cells compared to control endometrium. A mature form of IL-37b (IL-37bΔ1-45) effectively suppresses the migration and invasion of endometrial cancer cells by decreasing the expression of matrix metalloproteinase 2 (MMP2) via the Rac1/NF-κB signal pathway. This results in attenuated tumor metastasis in a peritoneal metastatic xenograft model of endometrial cancer.

Xishuang Wang
Zengtao Wei
Xiaoyan Wang
Research22 Mar 2021
Laboratory Investigation

Volume: 101, P: 760-774
Bacteria-induced expression of the pig-derived protegrin-1 transgene specifically in the respiratory tract of mice enhances resistance to airway bacterial infection

Fang Zeng
Chengcheng Zhao
Zhenfang Wu
ResearchOpen Access29 Sept 2020
Scientific Reports

Volume: 10, P: 1-11
A fixation method for the optimisation of western blotting

Jing Xu
Hebin Sun
Weijie Dong
ResearchOpen Access30 Apr 2019
Scientific Reports

Volume: 9, P: 1-10
The first set of universal nuclear protein-coding loci markers for avian phylogenetic and population genetic studies

Yang Liu
Simin Liu
Shou-hsien Li
ResearchOpen Access24 Oct 2018
Scientific Reports

Volume: 8, P: 1-12

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Engineering eukaryotic transposon-encoded Fanzor2 system for genome editing in mammals

Author Correction: Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation

Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation

Engineered IscB–ωRNA system with expanded target range for base editing

Engineering a transposon-associated TnpB-ωRNA system for efficient gene editing and phenotypic correction of a tyrosinaemia mouse model

An RNA editing strategy rescues gene duplication in a mouse model of MECP2 duplication syndrome and nonhuman primates

Development of deaminase-free T-to-S base editor and C-to-G base editor by engineered human uracil DNA glycosylase

Identification of a novel ANO5 missense mutation in a Chinese family with familial florid osseous dysplasia

Engineered CRISPR-OsCas12f1 and RhCas12f1 with robust activities and expanded target range for genome editing

Adenine base editing-mediated exon skipping restores dystrophin in humanized Duchenne mouse model

C-JUN overexpressing CAR-T cells in acute myeloid leukemia: preclinical characterization and phase I trial

High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects

Development of miniature base editors using engineered IscB nickase

Enhanced pericyte-endothelial interactions through NO-boosted extracellular vesicles drive revascularization in a mouse model of ischemic injury

Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells

In vivo treatment of tyrosinaemia with hypercompact Cas12f1

Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors

IL-37bΔ1-45 suppresses the migration and invasion of endometrial cancer cells by targeting the Rac1/NF-κB/MMP2 signal pathway

Bacteria-induced expression of the pig-derived protegrin-1 transgene specifically in the respiratory tract of mice enhances resistance to airway bacterial infection

A fixation method for the optimisation of western blotting

The first set of universal nuclear protein-coding loci markers for avian phylogenetic and population genetic studies

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