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Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting

A high-throughput microfluidic method for the analysis of the secretion levels of large populations of immune cells allows for the identification of kinase-coding genes regulating interferon-gamma secretion by CD4⁺ T cells.

Mahmoud Labib
Zongjie Wang
Shana O. Kelley
Research27 Nov 2023
Nature Biomedical Engineering

Volume: 8, P: 263-277
A multi-center study on the predictors of different subtypes of hemorrhagic transformation of brain infarction after thrombolysis in atrial fibrillation patients presented with embolic stroke

Sherihan Rezk Ahmed
Mohamed G. Zeinhom
Mohamed Fouad Elsayed Khalil
ResearchOpen Access05 May 2025
Scientific Reports

Volume: 15, P: 1-14
A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles

Exosomal PD-L1 (exoPD-L1) is a biomarker predicting immunotherapeutic responses. Here the authors report NanoEPIC, a nanoscale cytometry platform that enables phenotypic sorting and exoPD-L1 profiling from blood plasma by using magnetic-activated ranking to differentiate exosomal subpopulations.

Kangfu Chen
Bill T. V. Duong
Shana O. Kelley
ResearchOpen Access11 Sept 2023
Nature Communications

Volume: 14, P: 1-15
A high-dimensional microfluidic approach for selection of aptamers with programmable binding affinities

Generating aptamers for use as affinity reagents in analytical applications is important, but SELEX, the standard method for aptamer generation, is unable to select for pre-defined binding affinities. Now, by combining efficient particle display, high-performance microfluidic sorting and high-content bioinformatics, the method ‘Pro-SELEX’ can afford the quantitative generation of aptamers with programmable binding affinities.

Dingran Chang
Zongjie Wang
Shana O. Kelley
Research05 Jun 2023
Nature Chemistry

Volume: 15, P: 773-780
Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting

Microfluidic quantitative immunomagnetic cell sorting efficiently recovers potent tumour-infiltrating lymphocytes from solid tumours by leveraging specific expression levels of target cell-surface markers.

Zongjie Wang
Sharif Ahmed
Shana O. Kelley
Research27 Jan 2022
Nature Biomedical Engineering

Volume: 6, P: 108-117
Isolation of tumour-reactive lymphocytes from peripheral blood via microfluidic immunomagnetic cell sorting

Potent circulating tumour-reactive lymphocytes can be isolated from peripheral blood at high yield and purity via microfluidic immunomagnetic cell sorting.

Zongjie Wang
Sharif Ahmed
Shana O. Kelley
Research10 Apr 2023
Nature Biomedical Engineering

Volume: 7, P: 1188-1203
Single-cell mRNA cytometry via sequence-specific nanoparticle clustering and trapping

Cell-to-cell variation in gene expression creates a need for techniques that characterize expression at the level of individual cells. Now, a technique for characterizing mRNA expression has been developed. The technique uses the intracellular self-assembly of magnetic nanoparticles to quantitate RNA levels at the single-cell level.

Mahmoud Labib
Reza M. Mohamadi
Shana O. Kelley
Research02 Apr 2018
Nature Chemistry

Volume: 10, P: 489-495
Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting

The expression of therapeutic protein targets in circulating tumour cells isolated from blood samples of patients with cancer can be tracked at the single-cell level by antibody-mediated magnetic labelling and microfluidic sorting.

Mahmoud Labib
Zongjie Wang
Shana O. Kelley
Research27 Jul 2020
Nature Biomedical Engineering

Volume: 5, P: 41-52
High-throughput genome-wide phenotypic screening via immunomagnetic cell sorting

Immunomagnetic cell sorting implemented in a microfluidic chip can perform loss-of-function CRISPR–Cas9-mediated phenotypic screening at higher throughput than fluorescence-activated cell sorting.

Barbara Mair
Peter M. Aldridge
Shana O. Kelley
Research23 Sept 2019
Nature Biomedical Engineering

Volume: 3, P: 796-805
Assessment of the in vitro anti-diabetic activity with molecular dynamic simulations of limonoids isolated from Adalia lemon peels

Amal M. El-Feky
Wael Mahmoud Aboulthana
Ahmed A. El-Rashedy
ResearchOpen Access14 Sept 2024
Scientific Reports

Volume: 14, P: 1-19
Single-cell analysis targeting the proteome

In this Review, the authors describe single-cell protein analysis technologies and assess their advantages and limitations. The emerging technologies presented have the potential to reveal new insights into tumour heterogeneity and therapeutic resistance, elucidate mechanisms of immune response and immunotherapy, and accelerate drug discovery.

Mahmoud Labib
Shana O. Kelley
Reviews17 Feb 2020
Nature Reviews Chemistry

Volume: 4, P: 143-158

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