Fig. 5: De novo biosynthesis of flavone C-glycosides in E. coli cell factory. | Communications Biology

Fig. 5: De novo biosynthesis of flavone C-glycosides in E. coli cell factory.

From: Pathway-specific enzymes from bamboo and crop leaves biosynthesize anti-nociceptive C-glycosylated flavones

Fig. 5

The artificial pathway contains tree modules including the naringenin precursor module, the flavonoid CYP450 decoration module, the C-glycosylation module. Details of multispecies genes involved are described in the Supplementary Table 6. a Engineered flavone-2-hydroxylases (F2H) from bamboo-related Gramineae family were used to evaluate their ability to produce Vit and Isovit. b The complete flavonoid P450 module were further assembled to produce Ori and Isoori. c De novo production of flavone C-glycosides. E. coli extracts were sampled 72 or 96 h after induction and analyzed by LC–MS. Target compounds were viewed by ion-extracted chromatogram. Standard samples were also analyzed for comparison.

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