Fig. 4: Primary and super-charged NK cells triggered GSLC death and decreased the number of GSLCs in spheroids. | Communications Biology

Fig. 4: Primary and super-charged NK cells triggered GSLC death and decreased the number of GSLCs in spheroids.

From: Infiltrating natural killer cells bind, lyse and increase chemotherapy efficacy in glioblastoma stem-like tumorospheres

Fig. 4

Primary (p) and super-charged (s) NK cells (blue) were treated (IL-2 or IL-2 + antiCD16 mAb) and added to GS025 (a, left c) and NCH421k (b, right c) tumorospheres (green) in different NK: GSLC ratios (5:1 and 2.5:1) for 48 h. 3D reconstructed images in blend mode and spot analysis images, are presented in upper and lower rows for each GSLCs after, respectively. NK cells were added to GSLC tumoropsheres in 5:1 ratio. Images were taken after 24 h of co-culture. The number of dead GSLCs per spheroid (c) were determined 48 h after the addition of NK cells. Data are presented as means ± SEM (n = 3, data points represent measurement for each healthy donor). Scale bar = 70 μm (a), 100 μm (b).

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