Fig. 3: AAV-CasRx mediated Gm19619 knockdown in high-fat diet (HFD) fed mice significantly improved the mice glucose metabolism. | Communications Biology

Fig. 3: AAV-CasRx mediated Gm19619 knockdown in high-fat diet (HFD) fed mice significantly improved the mice glucose metabolism.

From: Downregulation of hepatic lncRNA Gm19619 improves gluconeogenesis and lipogenesis following vertical sleeve gastrectomy in mice

Fig. 3

a Schema of the AAV-EFS-CasRx vector for Gm19619 knockdown and the experimental timeline. b The relative RNA levels of hepatic Gm19619, G6pc, and Pck1 in Gm19619 knockdown (CasRx-19×3) (n = 10) and control (CasRx-ctrl) (n = 7) mice. c The fasting blood glucose levels in CasRx-19×3 mice (n = 10) and CasRx-ctrl (n = 7) mice. d–f The GTT (d), ITT (e), and PTT (f) assay of CasRx-19×3 (n = 10, PTT n = 8) and CasRx-ctrl (n = 7) mice. g The western blot results of G6PC and PCK1 in CasRx-19×3 and control group mice livers (n = 3). Two-tailed unpaired t-test. Error bars represent the SEM. *P < 0.05, **P < 0.01, and ***P < 0.001.

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