Fig. 3: Cell-based assay using the split NanoLuc™ luciferase sensor.
From: Split luciferase-based assay to detect botulinum neurotoxins using hiPSC-derived motor neurons
a Protocol of the CB-assay using the BoNT sensor4. b, c Comparison of rBoNT/A (0) (red empty circles), rBoNT/A (red circles) and rBoNT/E (blue squares) potency in the BoNT sensor4 CB-assay. Data points are mean of n = 4 independent experiments in triplicate. b Graph shows concentration–response curves. c Comparison of the pEC50 of rBoNT/A and E calculated from curves above. Statistical two-tailed Student’s t-tests: *P < 0.05; **P < 0.01; ***P < 0.001; NS, P > 0.05 not significant (NS). Error bars show SEM.
