Fig. 3: Both RAGA GTP/GDP binding states inhibit CD47 and promote phagocytosis.
From: RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradation
a Immunoblot analysis of indicated proteins in pBabe empty vector, wild type or mutant RAGA overexpressed A549 cells. b Representative flow cytometry of CD47 protein expression on the cell surface. c Statistical analysis of cell surface CD47 expression (n = 4 per group). d Representative flow cytometry analysis of in vitro phagocytosis in macrophage and GFP-labeled A549 coculture system. Numbers in the outlined areas indicate the percentage of macrophages with phagocytosis. e Statistical analysis of the percentage of phagocytic macrophages cocultured with empty vector, wild type or mutant RAGA overexpressed A549 as in (d) (n = 4 per group). Data were from two or three independent experiments. Statistical data were presented as mean ± SD. P < 0.05 is considered statistical significance. * indicates P < 0.05; **indicates P < 0.01; and *** indicates P < 0.001.
