Fig. 1: The antitubercular compound CymA binds to Mtb ClpC2. | Communications Biology

Fig. 1: The antitubercular compound CymA binds to Mtb ClpC2.

From: ClpC2 protects mycobacteria against a natural antibiotic targeting ClpC1-dependent protein degradation

Fig. 1

a Structural formula of cyclomarin A (CymA). b Domain organisation of Mtb ClpC1 alongside its partial homologue ClpC2. ClpC1 NTD, N-terminal ___domain (cyan); AAA, ATPases Associated with diverse cellular Activities (shades of grey); MD, middle ___domain (black). The ClpC1 partial homologue, ClpC2, is coloured according to sequence homology to ClpC1 NTD: homologous region (cyan); non-homologous region (light blue). c, d Injection profiles and binding isotherms for ITC titrations of ClpC11–145 against CymA (c) and ClpC2 against CymA (d). The dissociation constant is indicated within each panel. Measurements were performed at 37 °C titrating aliquots of 50 μM ClpC11–145 (c) or ClpC2 (d) to 5 μM CymA. N and KD values are the mean ± standard deviation from three independent experiments.

Back to article page