Fig. 2: Cdh23 forms lateral clusters on GUV membranes.
From: Transient interactions drive the lateral clustering of cadherin-23 on membrane
a–f The fluorescence images of GUVs marking the pre-bleach, bleach, and post-bleach recovery regions at selected time points (upper panel), and the corresponding fluorescence recovery profile (normalized) (lower panel). a–c Are for GUVs anchored with Cdh23 EC1-10, Cdh23 EC 1-21, and Cdh23 EC1-27, respectively. d–f Are for the same GUVs anchored with Cdh23 EC1-10, Cdh23 EC 1-21, and Cdh23 EC1-27, respectively but after 1,6-HD treatment. The solid lines represent the fitting of the fluorescence recovery over time to the model (see methods). (g) PICUP on the clusters of Cdh23 EC1-27 on GUV membranes identifies only the monomer band in the silver stained SDS-PAGE. Middle panel is the repeat of Fig. 1b, indicating the relative position of the dimer band in red box. The upper inset shows the eGFP-fluorescent image of the representative GUVs used for PICUP.
