Fig. 3: Lateral clustering of Cdh23 on the membrane enhances the kinetics of cell-cell adhesion.
From: Transient interactions drive the lateral clustering of cadherin-23 on membrane
a Time-stamp bright-field images of cell-cell aggregations of HEK293 cells untransfected (1st column), transfected with Cdh23 EC1-10 (2nd column), Cdh23 EC1-27 (3rd column), and Cdh23 EC1-27 along with the treatment with 1,6-HD (4th column). Scale bar: 50 µm. b The time-dependent growth of the cell-cell aggregation area of HEK293 cells exogenously expressing Cdh23 EC1-27 (red), Cdh23 EC1-27 and treated with 1,6-HD (olive), Cdh23 EC1-10 (blue), and untransfected cells (black). The error bars represent the standard error of the mean (SEM) with N = 15 aggregates. All the individual data points are shown in the scattered plots in the supplementary file. c Time-stamp bright-field images of cell-aggregates of HeLa, HEK293, HaCaT, and A549 cells differentially expressing endogenous Cdh23. Scale bar: 50 µm. d Growth of cell-cell aggregation area (in µm2) with time for HeLa (red), HEK293 (Olive), HaCaT (black), and A549 (blue) cell lines. Error bars represent the standard error of the mean (SEM) for N = 15 aggregates. All the individual data points are shown in the scattered plots in the supplementary file.
