Fig. 8: PDK1 knock-out inhibits YAP expression and nuclear translocation.
From: 3-Phosphoinositide-dependent kinase 1 drives acquired resistance to osimertinib
a Western blot shows expression of total YAP, pYAP (S127), pYAP (S397) in osimertinib-sensitive NCI-H1975, resistant NCI-H1975-OsiR, NCI-H1975-OsiR-PDK1-/- and NCI-H1975-OsiR-PDK1++/++ cells, b Quantitation of pYAP (S127), pYAP (S397) and total YAP in NCI-H1975 isogenic cell lines, c Immunoflourescence images of nuclear translocation of YAP detected by immunostaining with pYAP (Tyr 357) antibody on osimertinib-sensitive and resistant cell lines as well as PDK1 knockout and re-expressing cells, d Quantitative analysis of nuclear YAP signals in four NCI-H1975 isogenic cell lines, e Active-YAP expression was evaluated by IHC using anti-active-YAP antibody on NCI-H1975 and NCI-H1975-OsiR xenograft tumors. f Active-YAP signals were quantitated from IHC sections by imageScopre using 15–20 10X images per group. g Level of YAP was determined from RPPA analysis of osimertinib-sensitive and resistant xenograft tumors (left panel) and osimertinib treated residual sensitive and resistant tumors (right panel).
