Fig. 1: Overt alterations in brain region metabolism and altered inter-regional functional connectivity in 16p11.2 DEL mice. | Communications Biology

Fig. 1: Overt alterations in brain region metabolism and altered inter-regional functional connectivity in 16p11.2 DEL mice.

From: 16p11.2 deletion mice exhibit compromised fronto-temporal connectivity, GABAergic dysfunction, and enhanced attentional ability

Fig. 1

a 16p11.2 DEL mice have decreased activity in the piriform (PirC, p = 0.008) and auditory (AudC, p = 0.014) cortex with increased metabolic activity in the medial septum (MS, p = 0.001) and mammillary body (MB, p = 0.014). The impact of 16p11.2 deletion on metabolism in these regions was not significantly influenced by sex, so the data are pooled for both sexes. Increased functional activity in the hippocampal subiculum (Sub) was found only in males (sex x genotype interaction: p = 0.03, ANOVA). *P < 0.05 and ***P < 0.001 difference from WT (ANOVA, main effect of genotype). #P < 0.05 difference from same sex WT (Tukey’s HSD). Box plots show median and interquartile range with “Tukey” whiskers. b Heatmap showing the altered inter-regional connectivity of regions with altered metabolism in 16p11.2 DEL mice, determined using PLSR. Red denotes inter-regional connectivity present in 16p11.2 DEL but not WT mice (VIP 95% CI > 1.0 in 16p11.2 DEL and <1.0 in WT). Light red denotes z > 1.96 and dark red z > 2.58 difference between genotypes. Blue denotes lost inter-regional connectivity in 16p11.2 DEL mice (VIP 95% CI < 1.0 in 16p11.2 DEL and >1.0 in WT). Light blue denotes z > 1.96 and dark blue denotes z > 2.58 difference between genotyes. Data for Sub show connectivity changes selectively found in 16p11.2 DEL male mice that are not found in 16p11.2 DEL female mice. n = 12 (6 male) mice/genotype. Prefrontal cortex: aPrL Anterior Prelimbic Cortex, FRA Frontal Association Area, DLO Dorsolateral Orbital Cortex, MO Medial Orbital Cortex, LO Lateral Orbital Cortex, mPrL Medial Prelimbic Cortex, IL Infralimbic Cortex, Cg1 Cingulate Cortex; Cortex: Ins Insular Cortex, Piri Piriform Cortex, RSC Retrosplenial Cortex, S1 Somatosensory Cortex; Thalamus: AM Anteromedial Thalamus, TRN Thalamic Reticular Nucleus, aRT Anterior Reticular Thalamus, AV Anteroventral Thalamus, MD Mediodorsal Thalamus, VL Ventrolateral Thalamus, VM Ventromedial Thalamus; Mesolimbic: AcbC Nucleus Accumbens Core, AcbSh Nucleus Accumbens Shell, VTA ventral Tegmental Area; Basal Ganglia: DLST Dorsolateral Striatum, VMST Ventromedial Striatum, GP Globus Pallidus, SNC Substantia Nigra pars Compacta, SNR Substantia Nigra pars Reticulata; Amygdala: BLA Basolateral Amygdala, CeA Central Amygdala, MeA Medial Amygdala; Auditory: IC Inferior Colliculus, MG Medial Geniculate, DB Septum/Diagonal Band of Broca, LS Lateral Septum, MS Medial Septum, HDB Horizontal Limb of DB, VDB Vertical Limb of DB; Hippocampus: DSub Dorsal Subiculum, DH CA1 Dorsal Cornu Ammonis 1, DH CA2 Dorsal Cornu Ammonis 2, DH Mol Dorsal Molecular Layer, DH DG Dorsal Dentate Gyrus, VH CA1 Ventral Cornu Ammonis 1, VH CA3 Ventral Cornu Ammonis 3, VH Mol Ventral Molecular Layer, VH DG Ventral Dentate Gyrus; Multimodal: Hab Habenula, MB Mammillary Body. Regional abbreviations are also shown in the supplemental material (Table S3).

Back to article page