Fig. 6: Restoring GPX4 expression antagonizes KLF11’s ability to promote ferroptosis and inhibit cell proliferation.
From: KLF11 regulates lung adenocarcinoma ferroptosis and chemosensitivity by suppressing GPX4
a–f qRT-PCR and western blot confirming the restored GPX4 expression in A549 and PC9 cells transfected with KLF11-NC and KLF11-OE. g–j Dose-toxicity curves showing the viability of A549 and PC9 cells transfected with KLF11-NC, KLF11-OE, KLF11-NC+GPX4, and KLF11-OE+GPX4 upon RSL3 or IKE treatment at the indicated concentrations for 24 h. k–n Lipid peroxidation was measured by flow cytometry after C11-BODIPY staining in KLF11-NC, KLF11-OE, KLF11-NC+GPX4, and KLF11-OE+GPX4 treatment by RSL3 or IKE. o Characteristic alterations of mitochondria in A549 cells treated with RSL3 or IKE analyzed by TEM after restored GPX4 expression, Scale bars: 50 nm. p, q GPX4 expression promotes the proliferation of A549 and PC9 cells inhibited by KLF11.
