Fig. 8: TrcR represses the expression of the operon alr1537-alr1540 involved in BMAA export. | Communications Biology

Fig. 8: TrcR represses the expression of the operon alr1537-alr1540 involved in BMAA export.

From: Global translational control by the transcriptional repressor TrcR in the filamentous cyanobacterium Anabaena sp. PCC 7120

Fig. 8

a EMSA testing the binding of TrcR and TrcR-L44P to the promoter region of alr1537. The promoter of all0854 (trcR) was used as a control. b Transcription levels of alr1537, alr1538, alr1539 and alr1540 quantified by qRT-PCR in WT, ΔtrcR and C-trcR with or without BMAA treatment. n = 3 biologically independent samples. The experiment was repeated three times for each sample. Data shown are the mean values ± S.D. c BMAA sensitivity of ΔtrcRΔalr1537, ΔtrcRΔalr1538, ΔtrcRΔalr1539, ΔtrcRΔalr1540 and ΔtrcRΔalr1537-40 tested in BG11 liquid medium containing different concentrations of BMAA. d Uptake of BMAA in WT, ΔtrcR and ΔnatAΔbgtA at indicated time points. e, f Quantification of BMAA uptake or secretion in WT, ΔtrcR, C-trcR, ΔtrcRΔalr1537, ΔtrcRΔalr1538, ΔtrcRΔalr1539, ΔtrcRΔalr1540 and ΔtrcRΔalr1537-40. e Amount of intracellular BMAA quantified at indicated time points after transferring into the BMAA-free medium. f Amount of BMAA secreted into the supernatant after transferring into the BMAA-free medium for 60 min. Data shown in (d), (e) and (f) are mean values ± S.D from triplicates.

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