Fig. 6: PANX1 regulates mitophagy through ATP-P2Y-mTOR pathway.
From: Pannexin 1 targets mitophagy to mediate renal ischemia/reperfusion injury
a, b Intracellular and extracellular ATP content of PANX1KD and HK2 cells in H/R 6 h and control (n = 5). c, d The expression of Phosphorylation of mTOR substrates in HK2 cells of PANX1KD or control treated with ATP or not under H/R condition (n = 5). e–h Protein levels of mTOR obtained from HK2 H/R cells treated with inhibitors (Suramin) or agonists (Diquafosol tetrasodium) of the P2Y2 receptor (n = 5). i–l The expression of mitophagy-related proteins in the mTOR pathway inhibitor (rapamycin) and activator (MHY1485) treated HK2 cells were measured in the indicated groups (n = 5). GAPDH served as the loading control. Results are presented as means ± SEM. P values from Student’s t test. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001.
