Fig. 1: Characterization of human plasma by size fractionation.
From: Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles
a Schematic diagram of plasma fractionation using size exclusion chromatography with 2 mL input plasma and 2 mL eluted volumes collected per fraction (FR). b Bar graphs of particle concentration (in black) measured by tunable resistive pulse sensing on left y-axis, and protein abundance (in red) measured by absorbance at 280 nm on right y-axis. X-axis indicates each fraction from size exclusion chromatography with area of color shown for FR14 (red), FR58 (blue), FR912 (green), FR1619 (purple), FR2326 (orange), and FR3033 (yellow) respectively. Data are analyzed using RStudio (v2023.06.1 + 524) and reported as means ± SD of three biological replicates. c Representative transmission electron microscopy images of particles in individual fractions; scale bar: 100 nm. d Pie chart of percent distribution of particles with corresponding size ranges: ≥ 100 nm (red), ≥ 50 nm & < 100 nm (blue), and < 50 nm (green) identified in each fraction measured by TEM. e Schematic of plasma fractions with color schemes for transcriptomic analysis.
