Fig. 4: Fibrotic kidney was effectively ameliorated in UUO kidneys after inhibiting ACSL4.

a The representative image (six visual fields for each sample analyzed) stained with HE, PAS and MTS of kidney sections from UUO, FA or sham-operated kidneys (control). Animals received ACSL4 inhibitor or vehicle as indicated. The tubular lesion and interstitial fibrosis were further presented in quantification. Scale Bar: 50 μm. n = 5 mice. b Western-blot of UUO-treated whole-kidney lysates analyzing the expression of fibronectin, collagen-I and α-SMA. Schematic representations of quantitative data of indicated proteins shown at the right panel. n = 5 mice. c Western blot analyzing the expression of indicated proteins in the FA-treated kidney samples. The relative abundance of the indicated protein expression was normalized by GAPDH (right panel). n = 5 mice. d The mRNA expression of fibronectin, collagen-I and a-SMA were analyzed by RT-qPCR in UUO and FA-treated kidneys. Representative images from three independent experiments are shown above. n = 5 mice. These data are calculated from more than three independent experiments. Data are presented as mean ± SEM. *P < 0.05, ns means no statistical significance.