Fig. 7: ACSL4 inhibition reduced profibrotic reaction in TGF-β-induced HK-2 cells. | Communications Biology

Fig. 7: ACSL4 inhibition reduced profibrotic reaction in TGF-β-induced HK-2 cells.

From: Inhibition of ACSL4 ameliorates tubular ferroptotic cell death and protects against fibrotic kidney disease

Fig. 7

a Western-blot analyzing the expression of profibrotic cytokines TGF-β, p-Smad2 and p-Smad3 in TGF-β-induce HK-2 cells. The relative abundance of the indicated protein expression was normalized by GAPDH. Schematic representation of quantitative data of indicated proteins (right panel). b The expressions of profibrotic cytokines TGF-β, CTGF, FGF2 and PDGFB mRNA were analyzed by RT-qPCR in TGF-β-induced HK-2 cells. Representative images from more than three independent experiments are shown above. Data are presented as mean ± SEM. *P < 0.05, ns means no statistical significance.

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