Fig. 6: m6A modification regulates primiR-6858 processing. | Communications Biology

Fig. 6: m6A modification regulates primiR-6858 processing.

From: METTL14-upregulated miR-6858 triggers cell apoptosis in keratinocytes of oral lichen planus through decreasing GSDMC

Fig. 6

Real-time PCR showing primiR-6858 or miR-6858 expression in HOKs infected with wild type/ mutant METTL14- (a) or METTL3- (b) lentivirus. c Real-time PCR showing primiR-6858 or miR-6858 expression in HOKs with shMETTL14-, wild type METTL14- or mutant METTL14-lentivirus as shown. d Real-time PCR analyses of primiR-6858 or miR-6858 expression in HOKs infected with shMETTL3-, wild type METTL3- or mutant METTL3-lentivirus as shown. e, f Primary miR-6858 or miR-6858 levels in HOKs with FTO- or shHNRNPA2B1-lentivirus determined by real-time PCR. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 vs corresponding control group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs corresponding shMETTL14 or shMETTL3 group. Data were expressed as means ± standard deviation. All experiments were carried out at least 3 times. Student’s t test (e and f) and one-way ANOVA (a, b, c and d) were used for statistical analysis.

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