Fig. 2: MUC1-C is necessary for WDR5 and RBBP5 expression. | Communications Biology

Fig. 2: MUC1-C is necessary for WDR5 and RBBP5 expression.

From: MUC1-C intersects chronic inflammation with epigenetic reprogramming by regulating the set1a compass complex in cancer progression

Fig. 2

a, b BT-549/tet-MUC1shRNA (a) and MDA-MB-468/tet-MUC1shRNA (b) cells treated with vehicle or DOX for 7 days were analyzed for WDR5 and RBBP5 mRNA levels by qRT-PCR. The results (mean ± SD of 3 determinations) are expressed as relative mRNA levels compared to that obtained for vehicle-treated cells (assigned a value of 1) (left). Lysates were immunoblotted with antibodies against the indicated proteins (right). c BT-549/CshRNA and BT-549/MUC1shRNA#2 cells were analyzed for WDR5 and RBBP5 mRNA levels by qRT-PCR. The results (mean ± SD of 3 determinations) are expressed as relative mRNA levels compared to that obtained for CshRNA cells (assigned a value of 1) (left). Lysates were immunoblotted with antibodies against the indicated proteins (right). d BT-549/MUC1shRNA#2 cells transfected with a tet-Flag-MUC1-CD vector were treated with vehicle or DOX for 7 days. Lysates were immunoblotted with antibodies against the indicated proteins. e BT-549 cells treated with vehicle or 5 μM GO-203 for 48 h were analyzed for WDR5 and RBBP5 mRNA levels by qRT-PCR. The results (mean ± SD of 3 determinations) are expressed as relative mRNA levels compared to that obtained for vehicle-treated cells (assigned a value of 1) (left). Lysates were immunoblotted with antibodies against the indicated proteins (right). f BT-549 cells grown as monolayers (2D) and as mammospheres (3D) were analyzed for expression of the indicated mRNA levels by qRT-PCR. The results (mean ± SD of 3 or more determinations) are expressed as relative mRNA levels compared to that obtained for 2D cells (assigned as value of 1)(left). Lysates were immunoblotted against the indicated proteins (right). g BT-549/tet-MUC1shRNA 3D mammosphere cells treated with vehicle or DOX for 7 days were analyzed for the indicated mRNA levels by qRT-PCR. The results (mean ± SD of 3 or more determinations) are expressed as relative mRNA levels compared to that obtained for vehicle-treated cells (assigned a value of 1) (left). Lysates were immunoblotted against the indicated proteins (right).

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