Fig. 6: BDNF treatment enhances injury-mediated decreases in network activity.

a Changes in burstlet rate (Hz). e = 92 for 0g 0B; e = 85 for 0g 50B; e = 91 for 30g 0B; e = 64 for 30g 50B. b Changes in Fano factor (A.U.). e = 266 for 0g 0B; e = 315 for 0g 50B; e = 265 for 30g 0B; e = 193 for 30g 50B. c Changes in local efficiency (A.U.). e = 268 for 0g 0B; e = 259 for 0g 50B; e = 243 for 30g 0B; e = 236 for 30g 50B. d Functional connectivity matrices used for calculating local efficiency. Shown are representative matrices at each timepoint for the control condition. Colorbar represents strength of cross-correlation. e Representative raster plots for each condition. 0g = no glutamate; 30g = 30 µM glutamate; 0B = no BDNF; 50B = 50 ng/ml BDNF. Data in a–d from N = 4 independent experiments. For a–c: y-axis indicates percent change from baseline (pre-injury). Top row compares pre-treatment to 0d post-treatment, and bottom row compares pre-treatment to 7d post-treatment. Plots on left indicate means with 95% CIs, and p values calculated by RM ANOVA followed by Tukey–Kramer multiple comparisons test, where *p < 0.05, **p < 0.01, ***p < 0.001 and indicates significant differences between timepoints within the same condition. Distributions on right with means (black squares) and 95% CIs (vertical lines) show comparisons between conditions and were calculated via estimation statistics with p values calculated directly from CIs. N indicates number of experiments, and e indicates number of electrodes.