Fig. 1: C7683 potently stabilizes both full-length and TKBD-LHR-RING Cbl-b proteins in DSF assays. | Communications Biology

Fig. 1: C7683 potently stabilizes both full-length and TKBD-LHR-RING Cbl-b proteins in DSF assays.

From: The co-crystal structure of Cbl-b and a small-molecule inhibitor reveals the mechanism of Cbl-b inhibition

Fig. 1

The chemical structure of C7683 is presented. C7683 stabilized (a) TKBD-LHR-RING and (b) full-length Cbl-b at 3 μM with ΔTm values of 10 ± 0.4 and 12 ± 0.2 °C, respectively. The stabilization effect of C7683 was concentration dependent (c, d) for both constructs at concentrations ranging from 0.01 to 100 µM. All experiments were performed in triplicates (n = 3).

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