Fig. 4: Drosophila microbes suppress apoptosis in the germarium but not in mid-oogenesis.
From: Microbes control Drosophila germline stem cell increase and egg maturation through hormonal pathways
a Representative images of DAPI-stained egg chambers in females cultured in M+ or M- conditions for 3 days. b The profiles of the most developed stages of egg chambers from females cultured in M+ (blue) or M- (gray) vials for 2 or 3 days. c Representative images of TUNEL-positive (arrow heads) germaria expressing UASp-p35 alone or UASp-p35 driven by NGT40; Nos-Gal4 (NN-Gal4) (NN-Gal4 > UASp-p35). d The profiles of TUNEL-positive germaria from females of the indicated genotype cultured in M+ or M- vials. Each gene was overexpressed (p35, DIAP) or knocked down (DCP-1RNAi) in germline cells by NN-Gal4. The number of germaria examined are shown on the bottom (white). e The number of stage 13/14 eggs per ovary (UASp-p35 or NN-Gal4 > UASp-p35) cultured in M+ or M- conditions. For statistical analysis, a Chi-square analysis is used for (a) and a Wilcoxon rank sum test is used in (e). ****P ≤ 0.001 and ***P ≤ 0.005, **P ≤ 0.01, n.s. nonsignificant (P > 0.05). Data are represented as mean ± standard deviation. f The profiles of the most developed stages of egg chambers (UASp-p35 or NN-Gal4 > UASp-p35) cultured in M+ (blue or pink) or M- (gray or green) conditions. The number of egg chambers examined are shown in the graph (b, f). Dotted line indicates 25% (b and f) or 40% (d) of y-axis. Scale bar = 100 μm in (a) and 10 μm in (c).
