Fig. 4: Characterization of the fatty acid binding site in XcAP-1. | Communications Biology

Fig. 4: Characterization of the fatty acid binding site in XcAP-1.

From: Acid phosphatase-like proteins, a biogenic amine and leukotriene-binding salivary protein family from the flea Xenopsylla cheopis

Fig. 4

a Hydrophobic binding site residues of XcAP-1 depicted as light blue sticks, with the palmitoleic acid molecule shown as green spheres. b Surface representation of the putative leukotriene binding pocket in XcAP-1. Substituted catalytic residues shown in sticks, and the SO4 atom represented as an orange sphere. The dotted circle highlights the solvent-accessible cleft near XcAP-1’s Lys31 and Arg78 residues. Surface representation of the putative leukotriene binding pocket of (c) XcAP-2 and (d) XcAP-3 based on the superposition of the AlphaFold model onto the XcAP-1 crystal model. e Superposition of the human prostatic acid phosphatase (PDB: 1ND6) onto the XcAP-1 crystal model, highlighting the clashes between the side chain of the human enzyme and the 16-carbon palmitic acid in its XcAP-1 binding mode. Nitrogen atoms represented in blue, and oxygen atoms in red.

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