Fig. 8: SH3RF2 contributed to DDP resistance in OC cells by inhibition of RBPMS.

a DDP-resistant A2780 cells were transfected with RBPMS siRNA and RBPMS expressions were evaluated by RT-qPCR (n = 3) and western blotting (n = 3) at 24 h after cell transfection. DDP-resistant A2780 cells with depletion of SH3RF2 were transfected with RBPMS siRNA for 24 h and exposed to 30 μM DDP. b Western blots of lysates from cells stained for RBPMS at 48 h after DDP treatment. n = 3. c MTT assays at 48 h after DDP treatment. n = 3. d Cell apoptosis were determined by flow cytometry following Annexin V-FITC/PI double staining at 48 h after DDP treatment. n = 3. e Caspase 3 activities at 48 h after DDP treatment. n = 3. f Comet assays at 48 h after DDP treatment. n = 3. g Immunofluorescence of γH2AX at 48 h after DDP treatment (Scale bars, 25 μm). n = 3. h DDP-resistant A2780 cells with depletion of SH3RF2 were co-transfected with RBPMS siRNA, pAP1-Ta-luc plasmids, and pRL-TK plasmids for 24 h and exposed to 30 μM DDP. Luciferase activity assays were performed at 48 h after DDP treatment. n = 3. i Schema of the potential mechanism of DDP resistance in OC cells. NS, no significance. Data are expressed as the mean ± SD. The p values were determined by one-way ANOVA.