Fig. 5: Comparison of recombinant LBA protein production between FDHY23 and LHP1044 under high-temperature conditions. | Communications Biology

Fig. 5: Comparison of recombinant LBA protein production between FDHY23 and LHP1044 under high-temperature conditions.

From: Coupling thermotolerance and high production of recombinant protein by CYR1N1546K mutation via cAMP signaling cascades

Fig. 5

a Expressions of recombinant LBA protein at 46 °C. Protein production was analyzed at 24 h, 36 h, 48 h, and 60 h by SDS-PAGE. b Growth curves during recombinant LBA production at 46 °C. The red and blue lines represent strains FDHY23 and LHP1044, respectively. c Recombinant protein yield elevation at 46 °C for the effect of CYR1N1546K mutation. The strain LHP1044-CYR1N1546K, which was constructed by introducing the CYR1 mutation into strain LHP1044, also showed higher LBA production at 46 °C compared to LHP1044. For growth curves, values were calculated as average±SD from three biological replicates.

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