Fig. 6: Gene expression profiles in metformin-treated oocytes in response to vitrification.

Fresh oocytes (F), vitrified oocytes (V), and vitrified oocytes pretreated with metformin (M) were collected for transcriptome sequencing analysis. a Differentially expressed genes in F, V, and M groups. b Cluster plots of differentially expressed genes in F, V, and M groups. c GSEA pathway enrichment analysis in V group vs M group. d GSEA lattice diagram in V group vs M group. e Heatmap analysis of mRNA expression levels of fatty acid elongation-related mRNA in F, V, and M groups. f GO enrichment analysis of core genes in fatty acid elongation pathway.