Fig. 7: Lipid profiles in metformin-treated oocytes exposed to vitrification.

Fresh oocytes (F), vitrified oocytes (V), and vitrified oocytes pretreated with metformin (M) were collected for lipidome analysis. a Differentially expressed lipids in F, V, and M groups. b Heatmap analysis of relative expression abundance of lipids in F, V, and M groups. c K-means cluster analysis of differentially expressed lipids in F, V, and M groups. d Pie chart analysis of FFA expression trends in V, and M groups compared with F group, respectively.