Fig. 1: CS particles stimulated CD4+ TRM cell emergence and expansion along with silicosis progression. | Communications Biology

Fig. 1: CS particles stimulated CD4+ TRM cell emergence and expansion along with silicosis progression.

From: Crystalline silica-induced recruitment and immuno-imbalance of CD4+ tissue resident memory T cells promote silicosis progression

Fig. 1

a Schematic showed CD45-APC-Cy7 antibody intravenous (i.v.) injection to distinguish tissue-resident or circulating leukocytes. Circulating leukocytes were labeled. b Flow cytometry (FC) analysis of pulmonary CD4+ TRM cells (CD45inject– CD44+) and TEM (CD45inject+ CD44+) cells. c Percentages and counts of the CD4+ TRM cells were compared at the indicated time points. W week. d Representative FC analysis of CD4+ TRM cells for CD69, CD103, and CXCR6 expression, respectively. e FC analysis of CD4+ TRM cells for CD69 and CD103 expression. f, g The graph showed percentages and counts of CD69+ CD103+ (f) and CD69+ CD103– subsets (g) in CD4+ TRM cells of saline or CS-treated mice at the specified time points. The bar graphs are the combined results of at least three independent experiments. Individual mice are plotted on the graphs, n = 5 biologically independent animals. Values are reported as the mean ± SD. P value was determined by one-way ANOVA followed by Tukey’s test.

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