Fig. 3: Piezo1 channel activation promotes LPS-induced inflammatory response in Caco-2 Cells via intracellular Ca2+ responses.

A qRT-PCR showing the expression of TNF-α and IL-6 in Caco-2 cells exposure to LPS, Yoda1 or LPS with Yoda1 for 24 h (n = 6). B TNF-α and IL-6 released from Caco-2 cells were measured by Elisa method (n = 3). C qRT-PCR and Elisa showing the expression of TNF-α and IL-6 in Caco-2 cells co-cultured with or without macrophages exposure to LPS, or LPS with Yoda1 for 24 h (n = 3). D Representative Western blots showing protein expression in control Caco-2 cells and cells primed with 100 μg/ml LPS after treatment without or with 10 μM Yoda1 for 24 h. E Effects of Yoda1 at different concentrations (0.2, 2, 10 and 50 μM) on Caco-2 cells (n = 3). F Live microscopy of Ca2+ influx (green) in Caco-2 cells stimulated with Yoda1 in the presence or absence of extracellular calcium. The summary of fluorescence intensity of Ca2+ influx in Caco-2 cells stimulated with Yoda1 in the absence (G) or presence (H) of extracellular calcium. I Summary of mean peak intracellular Ca2+ responses to 10 μM Yoda1 in Caco-2 cells with or without extracellular calcium (n = 3). P values were determined by unpaired two-tailed Student’s t test, *P < 0.05; **P < 0.01; ***P < 0.001. Data are mean ± SEM.