Fig. 1: CTSB accumulation in the urine of HUA patients, kidneys of HUA mice and proximal tubular epithelial cells.
From: Cathepsin B-dependent glycolysis contributes to reduced renal uric acid excretion in hyperuricemia
a The SUA levels of the control (n = 9) and HUA (n = 15) patients. b Spearman analysis between SUA and FEua in patients. c Total CTSB in 24-hour urine of control and HUA patients. d Spearman analysis between SUA and 24-hour urinary CTSB in patients. e Western blot of CTSB in the kidneys of HUA mice. f Statistical analysis of CTSB protein expression in HUA mice. g mRNA expression levels of CTSB in the kidneys of HUA mice. h Western blot of CTSB in the kidneys of uricase knockout HUA mice. i Statistical analysis of CTSB expression in uricase knockout HUA mice. j CTSB mRNA expression in the kidneys of uricase knockout HUA mice. k Immunofluorescence staining of CTSB in the kidneys of HUA mice. l Immunofluorescence staining of CTSB in the kidneys of uricase knockout HUA mice. m Western blot of CTSB in HK-2 cells after uric acid stimulation. o Statistical analysis of CTSB protein expression. p CTSB mRNA levels in HK-2 cells after uric acid stimulation. n Immunofluorescence staining for CTSB after treatment with uric acid in HK-2 cells. q Western blot of CTSB in MRTECs after uric acid stimulation. s Statistical analysis of protein expression. t CTSB mRNA levels in MRTEC cells after uric acid stimulation. r Immunofluorescence staining of CTSB in MRTEC cells after treatment with uric acid. Bars = 25 μm. n ≥ 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
