Fig. 4: Microglial CerS1 attenuates LPS-induced inflammation and restores plasticity in a neuron-microglia co-culture. | Communications Biology

Fig. 4: Microglial CerS1 attenuates LPS-induced inflammation and restores plasticity in a neuron-microglia co-culture.

From: Exercise improves depressive-like behavior in adolescent mice by regulating sphingosine and ceramide metabolism through microglial CerS1

Fig. 4

A, B QPCR analysis and ELISA analysis of IL-1β, IL-6 and TNF levels in primary microglia after overexpression of CerS1 (N  =  3 per group, one-way ANOVA. A F (2, 6) = 183.4/445.3/78.4; B F (2, 6) = 107.2/350.0). QPCR analysis and ELISA analysis of IL-1β, IL-6 and TNF levels in BV2 microglia after overexpression of CerS1 (N  =  3 per group, one-way ANOVA. C F (2, 6) = 135.9/161.9/132.6; D F (2, 6) = 192.9/308.0). E Microglia and neurons co-culture model diagrams. F Representative images of primary neurons morphology. Neurons (green) are stained with MAP2. Nuclei (blue) are stained with DAPI. Scale bar is 20 μm. G Analysis of neurons branches number (N  =  24 per group, one-way ANOVA, F (2, 69) = 12.93). H, I Representative western blot images showing the protein levels of BDNF, Synaptophysin and PSD95 (N  = 3 per group, one-way ANOVA, F (2, 6) = 11.72/16.37/25.17). Data are shown as the mean ± s.d. P  <  0.05 is statistically significant.

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