Fig. 8: Extracellular vesicles and exosome secretion by regenerating CLP-PEG-MPC and CLP-PEG implanted corneas. | Communications Biology

Fig. 8: Extracellular vesicles and exosome secretion by regenerating CLP-PEG-MPC and CLP-PEG implanted corneas.

From: Collagen analogs with phosphorylcholine are inflammation-suppressing scaffolds for corneal regeneration from alkali burns in mini-pigs

Fig. 8

a–c Greater amounts of Tsg101+ EVs (red staining) were present in the grafted regions, with the highest level of secretion in the CLP-PEG implants compared to CLP-PEG-MPC implants and unoperated controls. DAPI (blue staining) was used to identify the cell nuclei. d–f CLP-PEG implants also had the highest amount of Tsg101+, CD9+ exosomes represented by yellow staining. White scale bars, 200 µm. g–i High resolution TEM of the basement membrane confirms the increased release of exosomes from the epithelium into the stroma. Black scale bars, 2 µm. j–l High magnification of the inset yellow-boxed areas showing the presence of exosomes (white arrowheads) in the stroma. Yellow scale bars, 500 nm.

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