Fig. 5: Neural signaling research with the use of fCOAT generated focus stimulation.
From: Robust and adjustable dynamic scattering compensation for high-precision deep tissue optogenetics
a Targeted activation of calcium signaling in representative neurons. Red: Cal590 fluorescence signal. Green: the expression of the opsin ChR2 used. Neuron 1 is the target stimulation neuron, and the rest are neurons that follow the response. b Individual traces of Cal590 fluorescence in 13 different ChR2 neurons under 488 nm illumination, using extended focusing and speckle light fields. The red line: focus, the blue line: speckle. c Individual traces of Cal590 fluorescence in different ChR2 neurons under 488 nm illumination using speckle light fields. d Individual traces of Cal590 fluorescence in different ChR2 neurons under 488 nm illumination using focus light fields. e Speckle light activation of neuron 1 triggers estimation of functional connectivity of different neurons. Functional connectivity of pairs of neurons (orange line, green dots) centered on neuron 1 is determined by testing the statistical dependence of neuronal calcium activity. f Quantification of Synchronization Index and cluster number under speckle light illumination. g Focus light activation of neuron 1 triggers estimation of functional connectivity of different neurons. h Quantification of Synchronization Index and cluster number under focus light illumination.
