Fig. 6: Anti-human-TIGIT agonistic mAb (αTIGIT) enhances suppressive activity of naive Treg cells. | Communications Biology

Fig. 6: Anti-human-TIGIT agonistic mAb (αTIGIT) enhances suppressive activity of naive Treg cells.

From: Anti-human-TIGIT agonistic antibody ameliorates autoimmune diseases by inhibiting Tfh and Tph cells and enhancing Treg cells

Fig. 6

a Representative flow cytometry plots for Treg subtypes divided into CD25 and CD45RA staining of CD4+ T cells and each indicated fraction from HCs are shown. b Each Treg subset stimulation protocol is shown. c TIGIT and PD-1 expression in each Treg subtype before and after stimulation in a representative donor. d The proportion and MFI of TIGIT and PD-1 expression in each Treg subtype in unstimulated and stimulated HCs (n = 3) is shown. e The coculture protocol for stimulated Treg subsets and responder T cells is shown. f CellTrace violet (CTV) dilution, which was analysed by flow cytometry after gating on CTV-labelled responder T cells, and the suppression rate (g) was calculated as follows: (1−(proliferation of cocultured responder T cell)/(proliferation of cultured responder T cell alone)) × 100) (mean ± SEM) are shown. The P values were determined using an unpaired two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

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