Fig. 5: TrcR represses the trn operon expression. | Communications Biology

Fig. 5: TrcR represses the trn operon expression.

From: Global translational control by the transcriptional repressor TrcR in the filamentous cyanobacterium Anabaena sp. PCC 7120

Fig. 5

a, b Transcription levels of the selected genes (trn, allrt06, allrt16 and allrt02) quantified by qRT-PCR in WT, ΔtrcR and C-trcR. n = 3 biologically independent samples. The experiment was repeated three times for each sample. Data shown are the mean values ± S.D. c Schematic illustration of the trn operon. The cognate amino acid and the anticodon are used for the naming of tRNA. For example, Asp-tRNAGUC represents the tRNA whose cognate amino acid is Asp and the anticodon of which is GUC. The name and sites of fragments amplified by qRT-PCR in (a) were indicated below. The positions of −10 box (red letters) and a sequence TGTAGTAT (shaded background), similar to the consensus-binding site of TrcR are also indicated.

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