Fig. 5: RNA and protein codetection plots.

a Feature codetection waterfall plot of solid tissue biopsy cohort including five skin, three primary, and three metastatic melanoma samples (n = 11, biologically independent samples) and of b liquid biopsy cohort including PBMCs from three healthy donors (no digestion) and three immunotherapy-treated melanoma patients samples (n = 6, biologically independent samples). Plots show the percentages of cells with “RNA only”, “RNA and protein”, “protein only”, or “neither RNA nor protein” detection category. c Percentage of feature pairs in the solid and d liquid cohort that were predominantly assigned to one category based on a threshold of >50% of cells. Feature pairs where the threshold was not reached for either of the three categories were assigned to “other”.