Fig. 5: PANX1 regulates mitophagy during renal ischemia reperfusion injury process.

a, b Western blot measured the protein levels of mitophagy-related proteins in HK2 cells subjected to H/R for the indicated time points (n = 5). Results are presented as means ± SEM. P values from One-way ANOVA with Dunnett’s multiple comparisons test. c Representative images of fluorescence of PANX1 and TOM20 protein from H/R or control (Bar = 20 μm). d Laser confocal scanning microscopy for LC3 and mitochondrion staining (Bar = 20 μm). e–h Western blot indicated the levels of mitophagy-related proteins in PANX1KD and PANX1OE cells treated with H/R or normal (n = 5). Results are presented as means ± SEM. P values from Student’s t test. i, j Mitochondrial Keima (mt-Keima) analysis of mitophagy response in the process of hypoxia and reoxygenation (Bar = 20 μm) (n = 5). Results are presented as means ± SEM. P values from Student’s t test. k, l Western blot analysis of mitophagy-related proteins expression in mice subjected to renal I/R operation for the indicated time points (n = 5). Results are presented as means ± SEM. P values from one-way ANOVA with Dunnett’s multiple comparisons test. m, n The levels of mitophagy-related protein in the kidney of the indicated PANX1 knockdown mouse groups after I/R insult (n = 5). Results are presented as means ± SEM. P values from Student’s t test. GAPDH served as the loading control. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001.