Fig. 5: ESPs from adult S. mansoni activate p38 MAPK and ERK in the tegument of opposite sex worms and activation can be attenuated by SB203580 and U0126, respectively. | Communications Biology

Fig. 5: ESPs from adult S. mansoni activate p38 MAPK and ERK in the tegument of opposite sex worms and activation can be attenuated by SB203580 and U0126, respectively.

From: Schistosoma mansoni excretory-secretory products induce protein kinase signalling, hyperkinesia, and stem cell proliferation in the opposite sex

Fig. 5

a, c Adult male worms, or b, d adult female worms, were incubated for 1 h in (a, b) SB203580 (10 μM), or (c, d) U0126 (10 μM), or DMSO (vehicle for U0126), before being exposed to 24 h culture media containing ESPs (or not, control) from opposite sex adult worms for 15 min. Worm proteins were extracted and processed for western blotting with anti-phospho- p38 MAPK or -ERK antibodies. Blots were re-probed for actin as loading control. Vertical dotted lines on the blot images indicate non-adjacent lanes. Mean relative change in phosphorylation (±S.E.M.; n≥3 biological replicates) in worms was calculated, against control, unexposed and uninhibited, values (assigned a value of 1, dotted line) based on band intensity analysis after normalisation against actin; **p ≤ 0.01, and ***p ≤ 0.001 (ANOVA), when compared to control values, or between ESP exposed and inhibited (as shown).

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