Fig. 5: MUC1-C and WDR5 regulate common sets of genes involved in chronic activation of the NF-κB inflammatory response. | Communications Biology

Fig. 5: MUC1-C and WDR5 regulate common sets of genes involved in chronic activation of the NF-κB inflammatory response.

From: MUC1-C intersects chronic inflammation with epigenetic reprogramming by regulating the set1a compass complex in cancer progression

Fig. 5

a, b Soluble chromatin from (i) BT-549/tet-MUC1shRNA cells treated with vehicle of DOX for 7 days (a) and (ii) BT-549/CshRNA and BT-549/NF-κBshRNA cells (b) was precipitated with a control IgG, anti-JUN, anti-FOS and anti-ATF3. The DNA samples were amplified by qPCR with primers for the SET1A (left) and WDR5 (right) pELS regions. The results (mean ± SD of 3 determinations) are expressed as percent input. c GSEA of the RNA-seq datasets from MUC1-C- (left) and WDR5- (right) silenced cells using the HALLMARK TNFA SIGNALING VIA NFKB gene signature. d Schema of the TRAF1 gene with highlighting of pELS region that includes NF-κB and AP-1 motifs. Genome browser snapshot of ATAC-seq data from the TRAF1 pELS region in BT-549/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days (left). Chromatin was analyzed for accessibility by nuclease digestion (right). The results are expressed as % undigested chromatin (mean ± SD and individual values). e Soluble chromatin from BT-549/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days was precipitated with antibodies against the indicated proteins. The DNA samples were amplified by qPCR with primers for the TRAF1 pELS regions. The results (mean ± SD of 3 determinations) are expressed as percent input. f Soluble chromatin from BT-549/CshRNA and BT-549/JUNshRNA cells was precipitated with the indicated proteins. The DNA samples were amplified by qPCR with primers for the TRAF1 pELS. The results (mean ± SD of 3 determinations) are expressed as percent input.

Back to article page