Fig. 6: ICB plus AKT inhibition could prolong survival in EMThigh-AKT subtype.
a Comparison of anti-PD-L1 therapy response rate among the four EMT clusters in IMvigor210 cohort; Complete response (CR), Partial response (PR), Stable disease (SD), Progressive disease (PD). b Comparison of AKT pathway activity in EMThigh-AKT subtype patients with different immunotherapy responses in IMvigor210 cohort; CR/PR group, n = 19; SD/PD group, n = 95. c Comparison of AKT pathway activity with different immunotherapy responses using anti-PD-1 in GSE78220 cohort; CR/PR group, n = 15; PD group, n = 13. d Comparison of anti-CTLA-4 therapy response rate among the four EMT clusters in Van_allen+SRP067586 cohort; Response (R), Nonresponse (NR). e Comparison of ICB therapy response rate among the four EMT clusters in TCGA cohort. Response (R) and non-response (NR). f Comparison of AKT pathway activity in EMThigh-AKT subtype patients with different immunotherapy responses in TCGA cohort; R group, n = 646; NR group, n = 2278. g MK2206 could overcome anti-PD-L1 resistance in 4T1 xenograft tumors; n = 5 biological replicates. h MK2206 could overcome anti-PD-1 resistance in 4T1 xenograft tumors; n = 5 biological replicates. i MK2206 could overcome anti-CTLA-4 resistance in 4T1 xenograft tumors; n = 5 biological replicates. j MK2206 could overcome anti-PD-L1 resistance in B16-F10 xenograft tumors; n = 4 biological replicates for tumor burden assay; n = 5 biological replicates for survival assay. k MK2206 could overcome anti-PD-1 resistance in B16-F10 xenograft tumors; n = 5 biological replicates. l MK2206 could overcome anti-CTLA-4 resistance in B16-F10 xenograft tumors; n = 5 biological replicates. m MK2206 could overcome anti-PD-L1 resistance in mGSC intracranial xenograft tumors; n = 6 biological replicates. n MK2206 could overcome anti-PD-1 resistance in mGSC intracranial xenograft tumors; n = 6 biological replicates. o MK2206 could overcome anti-CTLA-4 resistance in mGSC intracranial xenograft tumors; n = 6 biological replicates. Error bars indicate SD; the Student’s t test or Kaplan-Meier analysis was used to analyze statistical significance between 2 groups; For (m–o), scale bar = 200 μm.
