Fig. 2: I5 and I6 affect gastric and pancreatic tumor cells oxygen consumption and mitochondrial functional properties.

a, b (upper panels) Representative plots showing mean ± SD of the oxygen consumption rate (OCR) calculated for untreated (Control) and I5- or I6-treated AGS, MKN45 and PANC1 cells (according to IC50, 24 h), normalized to total protein using a BCA kit (measured as BCA absorbance). Cells were treated with distinct inhibitors of mitochondrial function: O (oligomycin), F (FCCP), A (antimycin A), and R (rotenone). Continuous OCR values (pmoles/min/µg protein) are shown. a, b (bottom panels) Mean ± SD of measured and calculated mitochondrial function parameters (n = 3 biological replicates with 3 readings). *p < 0.05, **p < 0.01, ***p < 0.001, as determined by unpaired two-sided Student’s t-test.