Fig. 3: B cell transitional, naive, memory and antibody-secreting cell population frequency demonstrates differences between compartments that are mainly disease-independent. | Communications Biology

Fig. 3: B cell transitional, naive, memory and antibody-secreting cell population frequency demonstrates differences between compartments that are mainly disease-independent.

From: B cell heterogeneity in human tuberculosis highlights compartment-specific phenotype and functional roles

Fig. 3

a Representative flow plots of the canonical transitional (Trans), naive, memory (Mem) and antibody-secreting cell (ASC) B cell phenotypes derived from staining CD19+ B cells with CD27 and CD38 (cancer control in cyan, n = 8; patients with TB in red, n = 60). b The relative frequencies of these four B cell phenotypes were compared between blood and lung tissue compartments. c The frequency of CD69+ memory B cells was assessed (cancer control in cyan, n = 5; patients with TB in red, n = 25). d This analysis was extended to lung draining lymph nodes (LN, n = 17) from the TB cohort for comparison. A representative gating strategy for LN derived B cells is included as Supplementary Fig. 2. Statistical analyses were performed using the Mann–Whitney test between unmatched samples, the Wilcoxon test for matched/paired samples and a three-way Kruskal–Wallis comparison. P values are denoted by * ≤0.05; ** <0.01; *** <0.001 and **** <0.0001.

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