Fig. 4: Illustration of 3D genome compartment dynamics of EMT in stem cell differentiation and cancer models.
From: Regulating epithelial-mesenchymal plasticity from 3D genome organization
Stem cell model was derived from the differentiation of H1-hESC cells. H1 – H1 hESC, ME – mesendoderm, TRO – trophoblast-like cell, NPC – neural progenitor cell and MSC – mesenchymal stem cell. Cell lines are arranged by their EMT score. a Genome-wide view of A and B compartments, colored red and blue respectively, in H1-hESC and its differentiated cell lines. b Pie chart depicting the distribution of dynamic (gray) and stable (black) compartments across the cell lines at both epithelial and mesenchymal compartments. Heatmap representation of the compartment PC1 values are shown on the right of each pie chart. c Heatmap representation of PC1 and expression values of epithelial (right) and mesenchymal (left) genes in TRO (EMT score = −0.17) and MSC (EMT score = 0.62). IGV Snapshots of an epithelial (KRT19; 5.3 kilobase locus) and mesenchymal (COL5A2; 157 kilobase locus) gene demonstrating concordant compartment and expression dynamics is shown in the middle.
