Fig. 1: Experimental setups and stimulation sequence for awake chick fMRI.
From: Functional MRI of imprinting memory in awake newborn domestic chicks
a Imprinting cage. Newborn chicks were first exposed to a hollow plastic ball with a flickering red/blue light at a frequency of 5 Hz. b Custom-made restrainer and 7 T fMRI system. Awake chicks were placed in an MR-compatible tube. To immobilise non-invasively the animals, a beak holder was used to control the beak movements and blocks of plastelines were used to cover the ears and reduce head movements. To avoid body-part movements, animals were wrapped in paper tissue before fixating the head. Subsequently, the animal’s body was taped to the restrainer. c A sequence of the block design experiment paradigm. Visual stimuli were presented in blocks of 16 s followed by 24 s dark. During the ON blocks, the visual stimulus (red/blue light) flickered at a frequency of 5 Hz. All parts of this figure were created by the authors (M.B., E.L.).
