Fig. 6: Characterization of neutrophils in patients with herpes zoster.
From: Single-cell immune profiling and validation of PBMCs in the onset of and recovery from herpes zoster
a Five clusters of neutrophils were displayed according to marker gene expression levels. Uniform manifold approximation and projection (UMAP) presentation of the heterogeneous clusters of neutrophils. b The UAMP plot shows the distribution of the five subpopulations across HA, HP and RP group. c Box diagram of the relative frequencies of five subpopulations across HA, HP and RP group. d The heatmap shows the DEGs of five neutrophils subpopulations. e GO BP enrichment analysis for the DEGs of neutrophils in HP patients. The red terms represent upregulated terms, and the blue represent downregulated terms. P value was derived by a hypergeometric test. f Violin plot of subtype-specific marker genes of neutrophils, including GNGT2, GM2A, FGL2, IFIT3, RSAD2, ISG15, CXCL2, MMP8, CAMP, LTF, TUBA1B, MPO, PRTN3, ELANE, RPL12 and SOX4. g Network diagram of receptor-ligand interaction pairs between neutrophils and other cell types. Network nodes are cell types, network edge thickness is the total number of ligand-receptor pairs, and line color is consistent with ligand cell type. h Heatmaps showing the top 30 cell–cell interactions in the scRNA-seq dataset of T cells, as inferred by CellPhoneDB. i Representative cytometric gating strategy for identification of neutrophils. j Proportion of peripheral neutrophils in HP and HA groups.
