Fig. 7: CAV1 overexpression attenuates the cardioprotective effects of NLRC5 overexpression in mice post MI. | Communications Biology

Fig. 7: CAV1 overexpression attenuates the cardioprotective effects of NLRC5 overexpression in mice post MI.

From: Targeting NLRC5 in cardiomyocytes protects postinfarction cardiac injury by enhancing autophagy flux through the CAVIN1/CAV1 axis

Fig. 7

a Representative Western blots and corresponding quantification of p62, ATG12-ATG5, and LC3-II for autophagy proteins in hearts transfected with Ad5-Ctrl, Ad5-cTnT-NLRC5, Ad5-cTnT-CAV1, or Ad5-cTnT-NLRC5 + CAV1 at 14 days post-MI (dpi) (n = 5 each). b Quantitative analysis of LVEF, LVFS, LVID; d, and LVID; s in mice treated with Ad5-Ctrl, Ad5-cTnT-NLRC5, Ad5-cTnT-CAV1, or Ad5-cTnT-NLRC5 + CAV1 at 28 dpi (n = 8 per group). c, d Regional scar area and corresponding quantification in Ad5-Ctrl, Ad5-cTnT-NLRC5, Ad5-cTnT-CAV1, or Ad5-cTnT-NLRC5 + CAV1 hearts shown by Masson trichrome staining of heart tissue sections at 28 dpi (n = 6 each). Scale bar, 1 mm. e Schematic model of NLRC5 exerting cardioprotective effect by promoting CAVIN1/CAV1-mediated autophagy (figure drawn using BioRender). Data are presented as mean ± SD. a, b, d, 1-way ANOVA with Tukey multiple comparisons test. *P < 0.05, **P < 0.01, and ***P < 0.001. ns indicates not significant.

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