Fig. 6: ZEB1 in macrophages has no major direct effect on tumor cells but is important for CCL2- and CCL22-driven recruitment of CD8 + T cells.
From: Macrophages foster anti-tumor immunity by ZEB1-dependent cytotoxic T cell chemoattraction
a Confluence of KPC cells alone or co-cultured with LysMCtrl or LysMΔZeb1 BMDMs (n = 3). b Representative images at t = 28 h and quantification over time of KPC cell invasion into a scratch wound without or with co-culture of LysMCtrl or LysMΔZeb1 BMDMs (n = 3). c Confluence of KPC cells alone or with LysMCtrl or LysMΔZeb1 BMDM conditioned medium (CM) (n = 2 KPC CM, n = 3 LysMCtrl and LysMΔZeb1 CM). d Transwell migration assay of CD8 + T cells alone or towards LysMCtrl or LysMΔZeb1 BMDMs in absence or presence of recombinant CCL2 and CCL22 (left panel, n > 3) or absence or presence of anti-CCL2 and anti-CCL22 antibodies (right panel, n = 3). Means ± SD; *p < 0.05; **p < 0.01; ns: not significant; 2-way ANOVA.
