Fig. 4: Michaelis-Menten kinetics for Cas12 trans-cleavage activity for crRNA-15, -20 and -40.
From: Profiling crRNA architectures for enhanced Cas12 biosensing
A summary of Cas12 trans-cleavage kinetics for crRNA-15 (a) crRNA-20 (b) and crRNA-40 (c) with varying substrate concentration. The concentrations of Cas12, crRNA and DNA were 50 nM, 12.5 nM, and 1 nM respectively. d–f Michaelis-Menten plots generated from (a–c), respectively. The inset shows the fitted Michaelis-Menten parameters resulting in the best fit (Km = 2224 nM, 360 nM and 536 nM for crRNA-15, crRNA-20 and crRNA-40, respectively).
